Antibody molecules can often, but not always, recover from extensive denaturation by urea, guanidine and extremes of pH (see also Chapter 11) Polyclonal antisera generally require quite harsh conditions (6–8 M urea, 5–6 M guanidinehydrochloride, pH < 3 or > 10) before the antigen–antibody bond is disrupted (1930) Denaturation of proteins by urea and related substances Nature 126 328 – 330, 3 – 384 OpenUrl CrossRef ↵ Greene RF, Jr, Pace CN (1974) Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme,Protein denaturation with guanidine hydrochloride or urea provides a different estimate of stability depending on the contributions of electrostatic interactions O D Monera, C M Kay, and R S Hodges Author informationCopyright and License informationDisclaimer
Preparation And Extraction Of Insoluble Inclusion Body Proteins From Escherichia Coli Abstract Europe Pmc
